Dereplication and evaluation of the antinociceptive and anti-inflammatory activity of hydroethanolic extract of leaves from Campomanesia xanthocarpa O. Berg.

Campomanesia xanthocarpa popularly known as gabiroba is used as a medicinal plant for the treatment of inflammatory diseases, ulcers, among other uses, requiring studies to assist in proving these activities. In this study, the extract of leaves from C. xanthocarpa (CxHE) was submitted to assays of formalin-induced paw-licking, peritonitis induced by lipopolysaccharide and carrageenan-induced mechanical hyperalgesia tests. In chemical analysis, a preliminary phytochemical screening and the determination of phenol and flavonoid content were carried out, in addition to analysis by ESI-MS/MS system and HPLC-DAD system. The CxHE presented compounds such as tannins, triterpenoids, steroids and saponins and content of phenols (35.9 ± 1.3 GAE/g extract) and flavonoids (23.3 ± 2.1 mg EQ/g extract). Protocatechuic acid, gallic acid, ethyl gallate, quercetin, and 3-methyl epigallocatechin gallate, alpha and beta-amyrins were identified. CxHE at doses of 10-1000 mg/kg p.o. demonstrated anti-inflammatory and antinociceptive effects in all in vivo assays employed in this study. [Figure: see text].

Ozone against Pseudomonas aeruginosa biofilms in contact lenses storage cases.

Pseudomonas aeruginosa is associated with ocular infections such as keratitis. Multipurpose contact lens solutions can be used for washing, disinfection and storage of contact lenses, however, P. aeruginosa biofilm disinfection by this method is unsatisfactory. The present study aimed to investigate the effectiveness of ozonated water in reducing P. aeruginosa colony count. Lenses kept in storage cases were contaminated with P. aeruginosa and disinfected using ozonized water, chlorhexidine, ultrasound and multipurpose solutions. The multipurpose solutions and ultrasound methods reduced colony count from 1.17 to 1.63 log10 CFU/cm2 (92.93% to 97.31%), respectively, of P. aeruginosa biofilm cell viability when compared to the positive control. Both, ozonated water and chlorhexidine showed 7.42 log reduction in the number of viable cells of P. aeruginosa biofilm. As compared to chlorhexidine, ozonized water did not depose any known toxic residues, so that we recommend it as an alternative disinfectant solution for contact lenses storage cases.

Evaluation of antimicrobial and cytotoxic activities of plant extracts from southern Minas Gerais cerrado.

The antimicrobial activity of plant hidroethanolic extracts on bacteria Gram positive, Gram negative, yeasts, Mycobacterium tuberculosis H37 and Mycobacterium bovis was evaluated by using the technique of Agar diffusion and microdilution in broth. Among the extracts evaluated by Agar diffusion, the extract of Bidens pilosa leaf presented the most expressive average of haloes of growth inhibition to the microorganisms, followed by the extract of B. pilosa flower, of Eugenia pyriformis' leaf and seed, of Plinia cauliflora leaf which statistically presented the same average of haloes inhibitory formation on bacteria Gram positive, Gram negative and yeasts. The extracts of Heliconia rostrata did not present activity. Mycobacterium tuberculosis H37 and Mycobacterium bovis (BCG) appeared resistant to all the extracts. The susceptibility profile of Candida albicans and Saccharomyces cerevisiae fungi were compared to one another and to the Gram positive Bacillus subtilis, Enterococcus faecalis and the Gram negative Salmonella typhimurium bacteria (p > 0.05). The evaluation of cytotoxicity was carried out on C6-36 larvae cells of the Aedes albopictus mosquito. The extracts of stem and flower of Heliconia rostrata, leaf and stem of Plinia cauliflora, seed of Anonna crassiflora and stem, flower and root of B. pilosa did not present toxicity in the analyzed concentrations. The highest rates of selectivity appeared in the extracts of stem of A. crassiflora and flower of B. pilosa to Staphylococcus aureus, presenting potential for future studies about a new drug development.

Evaluation of attimicrobial and cytotoxic activties of plant extracts from souuthern minas gerais cerrado / Avaliacao das atividades antimicrobiana e citotoxica de extratos de plantas do Cerrado do Sul de Minas Gerais

The antimicrobial activity of plant hidroethanolic extracts on bacteria Gram positive, Gram negative, yeasts, Mycobacterium tuberculosis H37 and Mycobacterium bovis was evaluated by using the technique of Agar diffusion and microdilution in broth. Among the extracts evaluated by Agar diffusion, the extract of Bidens pilosa leaf presented the most expressive average of haloes of growth inhibition to the microorganisms, followed by the extract of B. pilosa flower, of Eugenia pyriformis' leaf and seed, of Plinia cauliflora leaf which statistically presented the same average of haloes inhibitory formation on bacteria Gram positive, Gram negative and yeasts. The extracts of Heliconia rostrata did not present activity. Mycobacterium tuberculosis H37 and Mycobacterium bovis (BCG) appeared resistant to all the extracts. The susceptibility profile of Candida albicans and Saccharomyces cerevisiae fungi were compared to one another and to the Gram positive Bacillus subtilis, Enterococcus faecalis and the Gram negative Salmonella typhimurium bacteria (p > 0.05). The evaluation of cytotoxicity was carried out on C6-36 larvae cells of the Aedes albopictus mosquito. The extracts of stem and flower of Heliconia rostrata, leaf and stem of Plinia cauliflora, seed of Anonna crassiflora and stem, flower and root of B. pilosa did not present toxicity in the analyzed concentrations. The highest rates of selectivity appeared in the extracts of stem of A. crassiflora and flower of B. pilosa to Staphylococcus aureus, presenting potential for future studies about a new drug development.

Foi avaliada a atividade antimicrobiana de extratos hidroetanólicos de plantas sobre bactérias Gram positiva, Gram negativa, leveduras, Mycobacterium tuberculosis H37 e Mycobacterium bovis pela técnica de difusão em Agar e microdiluição em caldo. Dentre os extratos avaliados pelo método de difusão em Agar, o extrato da folha de Bidens pilosa apresentou a mais expressiva média de halos de inibição de crescimento frente aos microrganismos, seguido pelo extrato da flor de B. pilosa, da folha e semente de Eugenia pyriformis, da folha de Plinia cauliflora que apresentaram estatisticamente a mesma média de formação de halos inibitórios sobre bactérias Gram positivas, Gram negativas e leveduras. Os extratos de Heliconia rostrata não apresentaram atividade. Mycobacterium tuberculosis H37 e Mycobacterium bovis (BCG) mostraram-se resistentes a todos os extratos. O perfil de sensibilidade dos fungos Candida albicans e Saccharomyces cerevisiae foram comparáveis entre si e entre as bactérias Gram positivas Bacillus subtilis, Enterococcus faecalis e Gram negativa Salmonella typhimurium (p > 0.05). A avaliação da citotoxicidade foi realizada sobre células C6-36 de larvas de mosquito Aedes albopictus. Os extratos de caule e flor de H. rostrata, folha e caule de P. cauliflora, semente de Anonna crassiflora e caule, flor e raiz de B. pilosa não apresentaram toxicidade nas concentrações avaliadas. Os maiores índices de seletividade foram apresentados pelos extratos de caule de A. crassiflora e flor de B. pilosa para Staphylococcus aureus, apresentando potencial para estudos como futuros candidatos a fármacos.